Left/right arrows keys step through all slides (both horizontal and vertical). Use the navigation-mode option to fine tune Reveal navigation behavior: Navigation Mode If you wish you can also configure slide navigation to nest multiple slides within a single top-level slide to create a vertical stack. Reveal uses classic linear slide navigation by default. Note that the parallax-background-horizontal and parallax-background-vertical options are not required (the defaults shown above will be used if they are not specified). title : "Presentation" format : revealjs : parallax-background-image : background.png parallax-background-size : "2100px 900px" parallax-background-horizontal : 200 parallax-background-vertical : 50. The following example will fade in the text on the first step, turn it red on the second and partially fade out on the third: Multiple fragments can be applied to the same element sequentially by wrapping it. Turn text blue, then back to original on next step Turn text green, then back to original on next step Turn text red, then back to original on next step Here are all of the available fragment classes: Nameįades in, then out to 50% on the next step NOTE: These results are intended for research use only, and are not appropriate for use in clinical diagnosis nor for forensic purposes.# Slide 1 # Slide 2 # Slide 3 Slide up while fading in ::: Fragment Classes In order for this to work, we will need the excel sheet submitted by email as well as the paper copy. Sample names are imported from the electronic version of the ticket. If you use a NanoDrop to measure the concentration of your samples, remember to check the 260/230 ratio, and consider submitting samples at a higher concentration than the recommended 1-5 ng/μl and choosing “Do not dilute” on your ticket.ħ. The Fragment Analyzer detects RNA and DNA only. This is because the NanoDrop detects everything, including contaminants, in the sample. The NanoDrop frequently reads concentrations higher (sometimes ten-fold more than what the Fragment Analyzer shows). For best results on High Sensitivity RNA assays, submit samples with concentrations in the upper end of the concentration range.Ħ. If you are expecting a narrow peak(s), 10-50 bp wide, submit at concentrations between 0.5 and 1 ng/μl. As a rule, if you are expecting a broad peak(s), hundreds of bp wide, submit higher concentrations (up to 5 ng/μl). The High Sensitivity DNA Assays are designed to give the best results at about 1 ng/μl. If your sample is limited you can write “low”, or give your best estimate for the concentration if it is not low.ĥ. The main reason the concentration column is on the form is to ensure your chosen assay matches your sample concentrations. RNA, dsDNA, and ssDNA can be detected by the machine, and will be visible if they are in your sample no matter which assay you select.Ĥ. The Fragment Analyzer cannot run samples that contain dye, particularly glycogen-based dyes.ģ. Contaminants can create problems with the run, including a less intense signal. Samples turned in ahead of time will be stored appropriately until they are processed.Ģ. While pick-up occurs every day, DNA assays will run Mondays, Wednesdays, and Fridays, while RNA assays will be run Tuesdays and Thursdays. Guaranteed same-day service is not available. Plan for your results to be ready by the end of the next business day after your assay type is scheduled to run. (Available weekdays from 9AM to 5PM - email us if you leave or ship samples here).Īssay types are run on scheduled days (see below). Our shipping address for off-campus customers is Samples can be left in the labeled fridges at these locations Please email for a ticket or more information: Drop Off Locations: DNA Fragment Sizing: 25 bp to 48 Kb (assay dependent).Resolution as low as 3 bp (assay dependent).No minimum or maximum number of samples.Submission Requirements and Assay Features Fragment Analyzer Assay Information Available Assays Here are some FAQs about the Fragment Analyzer service. Here is an information sheet on how to assess RNA quality.Ī helpful guide from Agilent about the RIN/RQN number that applies to the Fragment Analyzer and Bioanalyzer.Īn explanation of The Significance of the 260/230 Ratio in Determining Nucleic Acid Purity Results are presented in a graphic format similar to a Bioanalyzer including size, concentration, molarity, and RIN values.ĭownload our introductory flyer to the Fragment Analyzer system! Samples are analyzed for concentration and molecular weight/size. The MBCL operates a Fragment Analyzer Service using the Advanced Analytical (Agilent) capillary electrophoresis system.
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